LTK- cells are mouse fibroblast cells

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Lab Report (CW1) FAQ’s
FAQ’s about LTK- cells.
What are LTK- cells? LTK- cells are mouse fibroblast cells. LTK- stands for lacking thymidine kinase.
I’m a little confused as to whether LTK means L cells that lack Thymidine Kinase or whether it means Leukocyte Tyrosine Kinase? Which ones is it? LTK- stands for lacking thymidine kinase.
Why are we using these cells? LTK- cells express beta-adrenergic receptors. If you use search for the two words highlighted in bold, you will be able to find literature with further information to help you with your report.
How much detail should I include on the LTK- cell line? This is up to you, but you need to provide enough detail to allow the reader to understand why you are using it.
Is it important to discuss in detail about the fact that LTK- cells lack thymidine kinase? No, the important points to understand are: 1. LTK- cells are mouse cells and so can be used safely on the bench; 2. LTK- cells express beta-adrenergic receptors.
I’m a little bit confused about the LTK- cells. Does this mean that normal fibroblast cells would use a tyrosine kinase pathway for cell growth, but they lack the enzyme to do so, therefore GPCR is the pathway they used? The cells are a mouse fibroblast cell line which can be used safely on the bench. The cells express beta adrenergic receptors. You should focus on the effects of the drugs themselves and the fact that LTK- cells express these receptors.
FAQ’s about the drugs used.
I was reading about how the drugs act on the G protein and how they affect cAMP, however it is not clear what this has to do with the actual cell proliferation. Please check the literature and lecture 2 for further information. You should think about what happens when cAMP increases in the cell.
According to the CW1 Workshop PowerPoint it says to write the function of the two drugs, such as the general effects GPCR drugs have on the body or do we have to explain about what it does to the beta-adrenergic receptor as well? These are two different subjects which should be explained separately, i.e. the physiological effects of the drugs on the body and secondly, the mechanism of action of the drugs.
FAQ’s about Data and data presentation.
Is necessary to include the table for cell culture observations or would it be better if I just made a graph? You should draw a graph of your data. The table describes changes in confluency during the experiment. You should include both. You must also include the table of cell counts provided in your schedule, so in total you should have two tables and one graph.
Should I include cell number calculations? Yes.
Do the figures, tables and references count towards the total word count? No. Read the CW1 assignment brief for further information on word count.
Other FAQ’s.
Do you have any recommendations for any books or websites that could be useful? To help you to understand G protein coupled receptor signalling, you should use the module text book and lecture notes as a starting point. There are many other references available which is your responsibility to source.
I was wondering how I would cite the lab schedule, if I used some information from there? Do I just write “Coventry University (2020)”, or maybe “Darrington, (2020)”. Is there a proper way to cite it? Both of these suggestions are incorrect. You obtained the material from the 4009BMS Moodle web page, so must include the hyperlink.
Good Luck from the 4009BMS team!

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